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update resume with PNAS citation
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resume.md

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## Publications
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<span class='skill paper hekstra'>**[MatchMaps: Non-isomorphous difference maps for X-ray crystallography](https://doi.org/10.1101/2023.09.01.555333)**, *bioRxiv (pre-print)*, 2023</span>
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<span class='skill paper hekstra'>**[MatchMaps: Non-isomorphous difference maps for X-ray crystallography](https://doi.org/10.1101/2023.09.01.555333)**, *bioRxiv (pre-print)*, 2024</span>
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<span class='skill paper hekstra'>**[Resolving conformational changes that mediate a two-step catalytic mechanism in a model enzyme](https://doi.org/10.1101/2023.06.02.543507)**, *bioRxiv (pre-print)*, 2023</span>
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<span class='skill paper hekstra'>**[Perturbative diffraction methods resolve a conformational switch that facilitates a two-step enzymatic mechanism](https://doi.org/10.1073/pnas.2313192121)**, *PNAS*, 2024</span>
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<span class='skill paper loria'>**[Uncovering the Molecular Interactions in the Catalytic Loop That Modulate the Conformational Dynamics in Protein Tyrosine Phosphatase 1B](https://pubs.acs.org/doi/10.1021/jacs.9b04470)**, *JACS*, 2019</span>
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##### Biochemistry & Biophysics
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- <span class='skill hekstra merck loria crews'>Protein expression and purification (IMAC, IEX, SEC) using FPLC and manual methods</span>
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- <span class='skill loria'>Enzyme kinetics assays in both steady-state and pre-steady-state regimes</span>
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- <span class='skill hekstra loria'>Enzyme kinetics assays in both steady-state and pre-steady-state regimes</span>
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- <span class='skill crews'>Immunoprecipitation (pulldown) to determine protein binding partners</span>
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- <span class='skill crews merck delfino'>Protein stability quantification via limited proteolysis, melting-point analysis, colorometric assays</span>
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- <span class='skill delfino'>Diazarine labelling for analysis of solvent-accessible surface area</span>

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